Dr. Shannon Servoss
Development of biomimetic materials based on poly-N-substituted glycine (peptoid) constructs for use in biomedical applications
1. Peptoids are easy and inexpensive to synthesize using an automatic peptide synthesizer, and can be designed to form extremely stable secondary structures. In addition, previous studies have shown that properly designed peptoids create a robust surface coating that resists the attachment of proteins and cellular debris. These structures take on various forms, such as micropores and microspheres, depending on side chain chemistry and the solvent. A coating that incorporates the various attributes of peptoids would be ideal for microarrays, as it would (i) allow for increased surface area for antibody immobilization, (ii) create a barrier between the glass slide and the antibody to retain function, (iii) be easy and inexpensive to synthesize, and (iv) minimize the background signal due to non-specific protein binding. Peptoids are also ideal candidates for use as an affinity reagent due to their inexpensive and facile synthesis, ability to incorporate unique reactive sites, highly stable helical structures, and potential for non-biofouling design. A peptoid designed for stability and with included reactive sites will serve as the support for an affinity reagent, essentially mimicking the constant region of an antibody. Reactive sites on the surface of the peptoid will be used to incorporate antigen binding peptides/peptoids.
2. While researchers are discovering many potential biomarkers for early stage cancer, validating these biomarkers for use in diagnostic systems is becoming increasingly difficult. The standard technique for validation is enzyme-linked immunosorbent assay, which requires two good affinity reagents against the biomarker. There are a limited number of affinity reagents currently available and the techniques for affinity reagent development are slow and expensive, greatly limiting the validation of biomarkers. Synthetic affinity reagents, such as affitoids, have the potential to be screened for much more rapidly than is currently possible. The rapid discovery of affinity reagents will ultimately lead to rapid validation of early stage breast cancer biomarkers, and thus a quicker path to a clinical diagnostic for early stage breast cancer.
Peptoids for Disease Detection and Treatment
Peptoids are non-natural peptide mimics that have the advantage of increased bioavailability and reduced immune response. The Servoss lab uses rational design to develop peptoids to be used in biomedical applications, including disease treatment and detection. Current work focuses on biosensors for virus and bacteria, drug delivery agents, tissue engineering, and Alzheimer’s disease treatment. For more details see sites.uark.edu/sservoss.